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Learn. I got the HI MEDIA Biochemical test results as follows: Citrate test +ve, Lysine +ve, Ornithine +ve, Urease +ve, Phenylalanine deamination -ve, Nitrate reduction -ve, H2S Production +ve, Glucose -ve, Adonitol-ve, Lactose -ve, Arabinose +ve, Sorbitol-ve. d. Arrangement most often observed is groups of 4, 6 & 8 from colonies on nutrient media. 7 years ago by Dr.E.I 0. Flashcards. Adequately recording the procedures and results of each test (25 pts): • I (or anyone else) ... Micrococcus luteus Micrococcus roseus Mycobacterium phlei Sporosarcina ureae Staphylococcus aureus Spell. I am unable to identify which Pseudomonas Spp. Created by. Micrococcus luteus. For strain 1, a slight decrease in turbidity is observed in the tube containing the bile salts (2nd from left), but the contents are almost as turbid as the control tube (far left); therefore, strain 1 … A positive motility test is indicated by a diffuse zone of growth flaring from the line of inoculation. Nitrate Reductase. A negative motility test is indicated by growth confined to the stab line. c. Arrangement usually in groups of 4; ferments maltose and mannitol; gelatinase positive. Organisms: Micrococcus ureae . Micrococcus luteus is a Gram-positive, to Gram-variable, nonmotile, spherical, saprotrophic bacterium that belongs to the family Micrococcaceae. Bacillus megaterium fermented sugars but didn't grow in the anaerobic area of the butt. Along with nutrients to support bacterial growth, it contains sodium thioglycollate, thioglycollic acid, L-cystine, methylene blue, and 0.05% agar. Micrococcus luteus are Gram-positive, to Gram-variable, motile -non motile, that are 0.5 to 3.5 micrometers in diameter and usually arranged in tetrads or irregular clusters. jmfanous. Sulfur can be reduced to H 2 S (hydrogen sulfide) either by catabolism of the amino acid cysteine by the enzyme cysteine desulfurase or by reduction of thiosulfate in anaerobic respiration. The “S” in SIM stands for sulfur. Match. Test. Gravity. Micrococcus. it is. SIM medium (Sulphide Indole Motility medium) which is a combination differential medium that tests three different parameters, Sulfur Reduction, Indole Production and Motility. It is urease and catalase positive. This would be read K/NC. Objective SIM is commonly used to differentiate members of Enterobacteriaceae. Micrococcus luteus uses the amino acids and does not grow in the butt of the slant. Micrococcus luteus - Survival in Amber. Micrococcus are Gram-positive cocci that are 0.5 to 3.5 micrometers in diameter and usually arranged in tetrads or irregular clusters. Enterobacter aerogenes fermented the sugars but turned to … M. luteus oxidizes carbohydrates to CO2 and water, and it does not produce acid from glucose as well as it does not make arginine dihydrolase or b-galactosidase. b. Urease Negative: Go to Section C.4.d. This would be read A/NC. A positive test for indole is denoted when a pink to red color band is formed at the top of the medium after addition of Kovacs Reagent. ... Isolate FAME (score) API BLAST results (similarity in base pairs) ... ygen requirements of Micrococcus, to test Morita’s hy- As the name suggests, it is commonly used to test a microbe for the ability to produce the gas hydrogen sulfide (H 2 S). first test Oxidative (unsealed medium turns yellow) Bacitracin. second test Susceptible to Bacitracin. Terms in this set (5) Glucose Oxidation-Fermentation. Write. Results of the bile solubility test are shown for two different strains of bacteria. Thioglycollate broth (Fluid Thioglycollate Medium) is a medium designed to test the aerotolerance of bacteria. They are generally strict aerobes and can generally reduce nitrate. STUDY. a. Urease positive: Go to Section C.4.c. They are generally strict aerobes and can generally reduce nitrate. Micrococcus luteus Microbio Lab. 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